Boemo bo phahameng ba boleng ba lijo tsa Alpha-Galactosidase CAS 9025-35-8 Alpha-Galactosidase phofo
Tlhaloso ea Sehlahisoa
α-galactosidase ke enzyme ea lelapa la glycoside hydrolase 'me e ameha haholo ho hydrolysis ea li-bond tsa galactosidic. Lintho tsa mantlha tsa 'mele le lik'hemik'hale tsa li-enzyme li hlahisoa ka tlase:
1. Lintho tsa 'mele: Boima ba molek'hule: Boima ba molek'hule ba α-galactosidase bo tloha ho 35-100 kDa. pH botsitso: E na le botsitso bo botle tlasa maemo a asiti le a se nke lehlakore, mme mofuta o loketseng wa pH hangata o pakeng tsa 4.0-7.0.
2.Ho tsitsa ha mocheso: α-galactosidase e na le botsitso bo botle ka boleng bo loketseng ba pH, hangata ka har'a 45-60 ° C.
3.Substrate specificity: α-galactosidase haholo-holo catalyzes hydrolysis ea α-galactosidic bonds le ho lokolla α-galactosidically e amanang le galactose ho tloha substrate. Li-substrates tse tloaelehileng tsa α-galactoside conjugation li kenyelletsa fructose, stachyose, galactooligosaccharides, le raffinose dimers.
4.Li-inhibitors le li-accelerator: mosebetsi oa α-galactosidase o ka 'na oa angoa ke lintho tse itseng: Li-inhibitors: Li-ion tse ling tsa tšepe (tse kang lead, cadmium, joalo-joalo) le li-reagents tse itseng tsa lik'hemik'hale (tse kang li-chelator tse boima) li ka thibela mosebetsi oa α- galactosidase.
5.Bahlahisi: Li-ion tse ling tsa tšepe (tse kang magnesium, potasiamo, joalo-joalo) le metsoako e itseng (e kang dimethyl sulfoxide) e ka ntlafatsa mosebetsi oa α-galactosidase.
Mosebetsi
α-Galactosidase ke enzyme eo mosebetsi oa eona o ka sehloohong e leng ho hydrolyze bond ea α-galactosidase le ho khaola sehlopha sa α-galactosyl ho ketane ea khabone ho hlahisa limolek'hule tsa α-galactose tsa mahala. Mesebetsi ea α-galactosidase e bontšoa haholo-holo likarolong tse latelang:
1.E thusa ho sila galactose lijong: Meroho, linaoa le lijo-thollo li na le alpha-galactose e phahameng, tsoekere eo batho ba bang ba thatafalloang ho e sila. Alpha-galactosidase e ka thusa ho theola alpha-galactose lijong le ho khothaletsa tšilo ea lijo le ho monya ha eona. Sena se bohlokoa haholo ho batho ba nang le bothata ba alpha-galactose kapa ba nang le mamello ea lactose.
2.Thibela ho ruruha le ho senya lijo: Nakong ea tšilo ea motho, haeba α-galactose e ke ke ea senyeha ka ho feletseng, e tla kena ka har'a kolone ebe e lomosoa ke libaktheria tse hlahisang khase ka maleng, tse bakang flatulence le indigestion. Alpha-galactosidase e ka thusa ho theola alpha-galactose le ho fokotsa ho hlaha ha litlamorao tsena tse mpe.
3.E khothalletsa kholo ea li-probiotics: Alpha-galactosidase e ka khothalletsa kholo ea li-probiotics ka maleng. Libaktheria tsena tse molemo li thusa ho boloka bophelo bo botle ba mala le ho leka-lekanya microbiome. Ka ho pshatla alpha-galactose lijong, alpha-galactosidase e fana ka matla le limatlafatsi tse hlokahalang ho hola.
4. Likopo tsa ho sebetsa ha lijo: Alpha-galactosidase e boetse e sebelisoa haholo indastering ea ho lokisa lijo, haholo-holo tlhahiso ea lihlahisoa tsa soya. Linaoa li na le palo e kholo ea alpha-galactose. Tšebeliso ea alpha-galactosidase e ka fokotsa litaba tsa alpha-galactose linaoang le ho ntlafatsa sebopeho le tatso ea lijo. Ka kakaretso, α-galactosidase e sebetsa haholo-holo ka hydrolyzing α-galactosidase bond. Mesebetsi ea eona e kenyelletsa ho thusa ho cheka galactose lijong, ho thibela khase le ho senyeha ha lijo, ho khothalletsa kholo ea li-probiotics le tšebeliso ea eona ho sebetsa lijo.
Kopo
Alpha-galactosidase ke enzyme e sebelisoang haholo-holo libakeng tse kang lijo tse lokisitsoeng le tlhahiso ea biofuel. Tse latelang ke ts'ebeliso ea eona liindastering tse fapaneng:
1.Indasteri ea lijo: α-galactosidase e ka sebelisoa ha ho etsoa lihlahisoa tsa soya, tse kang lebese la soya, tofu, joalo-joalo Sena ke hobane linaoa tse ling li na le alpha-galactose, tsoekere e thata hore 'mele o silehe 'me e ka khona habonolo. ho baka ho qhitsa le ho se thabe. Alpha-galactosidase e ka theola tsoekere ena eo ho leng thata ho e sila le ho thusa 'mele ho e sila le ho e monya hantle.
2.Indasteri ea ho fepa: Ho rua liphoofolo, lijo tsa aminoglycoside hangata li ruile ka α-galactose. Ho kenyelletsa α-galactosidase ho fepa ho ka thusa liphoofolo ho cheka tsoekere ena le ho ntlafatsa ts'ebeliso ea phepo e nepahetseng le ts'ebetso ea kholo ea liphoofolo.
Tlhahiso ea 3.Biofuel: Alpha-galactosidase e ka phetha karolo ea tlhahiso ea biofuel. Nakong ea phetoho ea li-biomass ho li-biofuel, li-polysaccharides tse ling tse setseng (tse kang galactose le oligosaccharides) li ka fokotsa katleho ea ho belisoa. Ho eketsa α-galactosidase ho ka thusa ho senyeha ha li-polysaccharides tsena, ho ntlafatsa katleho ea ho belisoa ha biomass le tlhahiso ea biofuel.
4.Indasteri ea tsoekere: Nakong ea tlhahiso ea tsoekere ea sucrose le tsoekere ea beet, li-polysaccharides tse setseng ka har'a bagasse le beet pulp hangata li kopana le tsona. Ho eketsa alpha-galactosidase ho potlakisa ho senyeha ha li-polysaccharides tsena, ho eketsa chai le katleho ea ts'ebetso ea tlhahiso ea tsoekere.
5.Lebala la Meriana: Alpha-galactosidase e boetse e sebelisoa litekong le liphekolong tse ling. Mohlala, mafung a mang a sa tloaelehang a lefutso, bakuli ba haelloa ke ts'ebetso ea alpha-galactosidase, e lebisang ho bokelleng ha lipid le matšoao a amanang. Tabeng ena, ho tlatselletsa α-galactosidase e sa tloaelehang ho ka thusa ho theola lipids tse bokelletsoeng le ho fokotsa matšoao a lefu.
Lihlahisoa tse Amanang:
Feme ea Newgreen e boetse e fana ka li-Enzymes ka tsela e latelang:
Bromelain ea boemo ba lijo | Bromelain ≥ 100,000 u / g |
Mokhahlelo oa lijo oa alkaline protease | Alkaline protease ≥ 200,000 u / g |
Papain ea boemo ba lijo | Papain ≥ 100,000 u / g |
Lijo tsa boemo ba laccase | Laccase ≥ 10,000 u/L |
Mofuta oa acid oa protease oa mofuta oa APRL | Acid protease ≥ 150,000 u / g |
Cellobiase ea boemo ba lijo | Cellobiase ≥1000 u / ml |
Li-enzyme tsa dextran tsa boemo ba lijo | Dextran enzyme ≥ 25,000 u / ml |
Lipase tsa boemo ba lijo | Lipase ≥ 100,000 u / g |
Protease ea boemo ba lijo | Protease e sa nke lehlakore ≥ 50,000 u / g |
Mofuta oa lijo oa glutamine transaminase | Glutamine transaminase≥1000 u/g |
Mofuta oa lijo tsa pectin lyase | Pectin lyase ≥600 u/ml |
Pectinase ea boemo ba lijo (metsi a 60K) | Pectinase ≥ 60,000 u / ml |
Catalase ea boemo ba lijo | Catalase ≥ 400,000 u / ml |
Sekhahla sa glucose oxidase | Glucose oxidase ≥ 10,000 u / g |
Sehlopha sa lijo sa alpha-amylase (e hanana le mocheso o phahameng) | Mocheso o phahameng oa α-amylase ≥ 150,000 u / ml |
Sehlopha sa lijo sa alpha-amylase (mocheso o mahareng) Mofuta oa AAL | Mocheso o mahareng alpha-amylase ≥3000 u / ml |
Alpha-acetyllactate decarboxylase ea boemo ba lijo | α-acetyllactate decarboxylase ≥2000u/ml |
Boemo ba lijo β-amylase (metsi 700,000) | β-amylase ≥ 700,000 u / ml |
Mofuta oa lijo oa mofuta oa β-glucanase BGS | β-glucanase ≥ 140,000 u/g |
Protease ea boemo ba lijo (mofuta oa endo-cut) | Protease (mofuta o sehiloeng) ≥25u/ml |
Mofuta oa lijo oa xylanase XYS | Xylanase ≥ 280,000 u / g |
xylanase ea boemo ba lijo (acid 60K) | Xylanase ≥ 60,000 u / g |
Mofuta oa GAL oa mofuta oa glucose amylase | Enzyme ea tlhaho≥260,000 u/ml |
Mokhahlelo oa lijo Pullulanase (metsi a 2000) | Pullulanase ≥2000 u / ml |
Cellulase ea boemo ba lijo | CMC≥ 11,000 u/g |
Cellulase ea boemo ba lijo (karolo e felletseng ea 5000) | CMC≥5000 u/g |
Alkaline protease ea boemo ba lijo (mofuta o nang le ts'ebetso e phahameng) | Mosebetsi oa alkaline protease ≥ 450,000 u / g |
Glucose amylase ea boemo ba lijo (100,000 e tiileng) | Mosebetsi oa Glucose amylase ≥ 100,000 u / g |
Protease ea boemo ba lijo (e tiileng 50,000) | Mosebetsi oa protease ea acid ≥ 50,000 u / g |
Protease ea boemo ba lijo e sa nke lehlakore (mofuta o nang le ts'ebetso e phahameng) | Ts'ebetso ea protease e sa nke lehlakore ≥ 110,000 u / g |